By E. E. Hansen, J. F. Hubstenberger, G. C. Phillips (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
Twenty-seven chapters care for the regeneration of vegetation from protoplasts and genetic transformation in quite a few species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. those reports replicate the far-reaching implications of protoplast know-how in genetic engineering of crops.
This quantity is of designated curiosity to complicated scholars, lecturers, and learn scientists within the box of plant tissue tradition, molecular biology, genetic engineering, plant breeding, and basic plant biotechnology.
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Extra resources for Plant Protoplasts and Genetic Engineering VII
Arabica caturra, C. canephora RS, MS mod. S M C. arabica typica / MS mod. S M C. S M Culture medium of donor tissue Species/donor tissue Table 1. Isolation, culture, and regeneration of coffee protoplasts Schopke et al. (1987), Schopke (1989) Schopke (1989) Acuna and de Pena (1991) Sprial and Petiard (1992) Tahara et al. (1994) Barton et al. (1992), Adams and Zarowitz (1994) IS 13 >8 b 6-12 >6b ND BS mod. 3 ~M BS mod. 3 ~M Blaydes mod. 3 ~M MS mod. + BAP S ~M coconut water 10% Complex medium + kinetin I 0 ~M Yasuda et al.
Based on these results, NH 4N0 3 was eliminated completely from the media. In fact, the ammonium ion is generally considered as a critical factor for protoplast culture. It is necessary for leaf protoplasts in tobacco species (Meyer 1985) but, in contrast, an inhibitory effect has been reported in other species (Okamura et al. 1984; Tremouillaux-Guiller et al. 1987). Ochatt et al. (1988) reported that the omission of NH 4N0 3 improved protoplast regeneration in woody species. Based on these results, ammonium was eliminated completely from MT' media.
Canephora and Arabusta. Concentrations are given in mgll. 5 5 30000 30000 20000 92000d 20000 92 000 30000 10 000 aMurashige and Skoog (1962). bBiaydes (1966). cYasude et al. (1985). dGradually reduced to 18 000; see text. 5 M mannitol. 5. The dishes are agitated on a rotary shaker at 50 rpm at 26°C. 3. After 6 h, sieve the suspension through a 25-llm nylon mesh and centrifuge at 100 g for 5 min. Wash the pelleted protoplasts twice with medium (3) and culture them at 26°C in darkness at a density of 2 x 105Iml in 3 ml of medium (3) in 3-cm Petri dishes.