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Takeuchi, N. Localized action of gamma-aminobutyric acid on the crayfish muscle, J. Physiol. 177, 225-238 (1965). 32 A. Takeuchi Takeuchi, A. & Takeuchi, N. Anion permeability of the inhibitory post-synaptic membrane of the crayfish neuromuscular junction, J. Physiol. 191, 575-590 (1967). Takeuchi, A. & Takeuchi, N. A study of the action of picrotoxin on the inhibitory neuromuscular junction of the crayfish, J. Physiol. 205, 377-391 (1969). A Molecular Mechanism for the Action of Benzodiazepines on GABAergic Transmission A.

Modulators were purified 500-fold: 5^ug was used for GABA binding and 30 /-g for BZD binding. * Undetectable — not determined. Studies on C6 glioma cells and on NB2A neuroblastoma cell lines have been particularly useful to establish that, indeed, GABA receptor sites and the protein "modulator" are located on the same cells. As shown in Table 2, C6 glioma and neuroblastoma cells possess a significant amount of specific, saturable and high + affinity recognition sites for GABA. The number of Na independent binding sites for GABA present in these cell membranes is increased by freezing, thawing and Triton X-100 treatment.

Takeuchi, N. Localized action of gamma-aminobutyric acid on the crayfish muscle, J. Physiol. 177, 225-238 (1965). 32 A. Takeuchi Takeuchi, A. & Takeuchi, N. Anion permeability of the inhibitory post-synaptic membrane of the crayfish neuromuscular junction, J. Physiol. 191, 575-590 (1967). Takeuchi, A. & Takeuchi, N. A study of the action of picrotoxin on the inhibitory neuromuscular junction of the crayfish, J. Physiol. 205, 377-391 (1969). A Molecular Mechanism for the Action of Benzodiazepines on GABAergic Transmission A.

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