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Since the responses need to be singularly and unambiguously assigned to each event, the interstimulus time needs to be at least as long as the typical width of the BOLD response curve (8–10 s). Therefore, these experiments need to be very long, and become prohibitively long for certain subject populations such as children and the elderly. When using a constant interstimulus interval (ISI), such as might be used in a block-design paradigm, the optimal ISI is about 10–12 s. Dale101 showed that responses to visual stimuli, presented as rapidly as once every 1 s, can be adequately separated using overlap correction methods.
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