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Specific Reactivity of Monoclonal Antibodies to Cancer Tissues A monoclonal antibody of HF10B4 clone was tested for reactivity with lung adenocarcinoma tissues from patients. with all seven adenocarcinoma As shown in Table 6, this MoAb reacted tissues. 59 Its reactivity against the normal GROWTH OF HYBRIDCMA CLONES IN SERUM-FREE MEDIUM* Cell density at day 5 (cells/ml X 10" 5) Doubling time in logarithmic phase (hr) serum free HF10B4 12 23 20 13 HF10F7 10 24 31 19 HB4G7 13 29 20 12 HB4C5 10 17 40 21 * Hybridoma clone cells were plated at a density of 1 0 5 cells per ml of serum-free or serum-containing medium (see text).
These HAT sensitive fusion partners were tested for the preparation of hybridomas using lymphocytes from human lymph nodes and peripheral blood. II. CHARACTERISTICS OF A FUSION PARTNER CELL LINE, HO-323 HO-323, derived from parental WIL2-NS cells, was resistant to 30 ug/ml of 6-thioguanine and died within one week when cultivated in HAT medium (Fig. 1). HO-323 doubles every 15 hr, has a cloning efficiency of 10% and can proliferate 3 well even when plated at low cell density of less than 1 x 10 cells/ml.
The optimal technological approach to the development of human hybridomas. Journal of Biological Response Modifiers, 4^ 213-239. 12. L. A. (1985). In vitro development-of human monoclonal antibody secreting plasmacytomas. Human Immunology, 13, 199-209. 13. G. (1985). for Trends in Biotechnology, 3_, 167-170. monoclonal antibody production. 14. , Larrick, J. and Raubitschek, A. ) (1985). Human Hybridomas and Monoclonal Antibodies. Plenum Press, New York. 15. James, K. T. (1987). duction: Human monoclonal antibody pro Current status and future prospects.