By Magdalena Trzcińska
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Extra resources for Kidney Transplantation - New Perspectives
Endothelial cells in allograft rejection. Alheim, M. et al. (2010). A flow cytometric crossmatch test for simultaneous detection of antibodies against donor lymphocytes and endothelial precursor cells. 269-277. Alkhunaizi, A. (2006).
2010). , 2008). In summary, solid-phase analysis represents a powerful tool for anti-HLA antibody analysis and has several advantages over other assays. First, due to its superior sensitivity and specificity, low concentrations of DSAs pre- and post-transplant can be detected. Second, this technology does not require viable cells. Third, it detects only anti-HLA antibodies. 8. , 2009). Historically, transplantation for such patients was possible only when a donor could be found whose tissues did not express any HLA that the recipient produced antibodies against.
In addition, the products of HLA-C locus genes are not expressed on platelets (Zemmour & Parham, 1992). During the last decade, several reports have addressed their role in clinical transplantation. , 2006). , 2006). These investigators have also discovered that, to produce a positive FCCM, the median fluorescence intensity (MFI) values of anti-HLA-C antibodies must be significantly higher that those produced by antibodies against HLA-A and –B. The considerable influence of anti-HLA-C antibodies on kidney allocation and graft outcomes has been noted in a recent review of Gebel and Bray (Gebel and Bray, 2010).