Download Human Hybridomas and Monoclonal Antibodies by Jerry W. Shay (auth.), Edgar G. Engleman, Steven K. H. PDF

By Jerry W. Shay (auth.), Edgar G. Engleman, Steven K. H. Foung, James W. Larrick, Andrew A. Raubitschek (eds.)

Soon after Kohler and Milstein defined using somatic cellphone hybridization for the creation of murine monoclonal antibodies of wanted specificity, this quite uncomplicated process turned generally utilized. certainly, construction of murine monoclonal antibodies is now thought of regimen by means of immunologists and nonimmunologists alike. despite the fact that, as heterologous proteins, mouse monoclonal antibodies have one significant dilemma: they're immunogenic in guy and, for that reason, their use in vivo is significantly constrained. An seen approach to this challenge is to provide human hybridomas with a similar concepts used for the creation of rodent hybrids. regrettably, the heritage of human hybridomas has been marked through substantive and infrequently exasperating tech­ nical difficulties, and the 1st stories of hybrids secreting human immu­ noglobulin of wanted specificity didn't seem till 1980. those stories have been met with preliminary enthusiasm, however it quickly turned obvious that whereas human lymphocytes should be fused, their frequency, point of Ig synthesis, and balance have been such that creation of human antibodies with this system was once neither regimen nor functional. still, a enough variety of investiga­ tors persisted, and through the subsequent five years fairly effective B-cell fusion companions in addition to better equipment of Epstein-Barr virus transformation have been built. iteration of human T -T hybrids has additionally been accomplished, even if difficulties of chromosomal balance stay a considerable hindrance, extra so than with B-cell lines.

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Herzenberg, L. , 1978, Cell hybrids of myelomas with antibody forming cells and T-lymphomas with T cells, in: Handbook of Experimental Immunology, 3rd ed. (Weir, D. ), F. A. Davis, pp. 7. Jongkind, ]. , 1982, Nonselective isolation of fibroblast heterokaryons, hybrids and cybrids by flow sorting, in: Techniques in Somatic Cell Genetics (J. W. ), Plenum Press, New York, pp. 81-100. HUMAN HYBRlDOMAS AND MONOCLONAL ANTIBODIES 19 Jongkind, j. , 1979, Isolation of human fibroblast heterokaryons with two-color flow sorting (FACS II), Exp.

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Lymphoblastoid cells (A) KR-4. (B) B6, and (D) KR-4 x B6 hybridomas show abundant polyribosomes, irregular nucleus, scant RER, prominent Golgi apparatus. Myeloma-like cells (C) KR-12 and (E) KR-12 x B6 hybrids show welldeveloped RER, scattered cytoplasmic vacuoles, and mitochondria (magnification 20,000 X). FIGURE hybrids (KR-12 X B6 and KR-4 X B6 cells) were grown as ascites in nude mice after a single passage as a solid subcutaneous tumor in irradiated (350 R) nude mice and then grown in vitro.

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