By Dr. B.S. Singh Et Al
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In the early 1980s, DNA regions (hypervariable mini satellite DNA) were discovered which varied in nucleotide sequences between individuals, so that no two individuals, except identical twins, had identical, hypervariable regions. , 1985). It is known that 99 per cent of base sequence is same in the DNA of all human beings. Only the sequence of very short stretches of DNA, sprinkled over the total DNA of a cell which is about three million base pairs, differs from person" to person. Of the total DNA, about one in 1000 base pairs is a site of variation in the Introduction ..........
Modification of enzymes by incorporating, a cofactor which is normally associated with a quite different type of enzymes. An example is the alkylation of a cysteine residue in the active site of papain with a flavin derivative transforming the hydrolase into an oxidoreductase (Duine, 1991). RECOMBINANT DNA TECHNOLOGY Recombinant DNA Technology provides molecular genetics to involve directed manipulation of genetic material and the transfer of genetic information between species which cannot interbreed.
In moths and butterflies. They do not infect vertebrates, other invertebrates, microorganisms or plants. In view ofthis restricted host range, baculoviruses cannot be used directly as vectors of immunogens for vaccination (either in man or in other vertebrates). , caterpillars). Therefore, not only are they safe to use (due to their restricted host specificity) but are also cost-effective as large quantities ofimm unogen s can be made. 28 ....................................................................................