By Sergei V. Konev
Fluorescence and phosphorescence are proving to be super delicate probes for elucidating conformation of proteins and nucleic acids and for learning molecular interactions. more recent instrumentation and methods pontificate nice promise for the way forward for those luminescence equipment in biopolymer learn. It needs to be famous, although, that the invention that sure amino acids, purines, and pyrimidines emit fluorescence or phosphorescence is comparatively fresh, taking place in the final decade. Professor Konev is likely one of the pioneers within the program of those systems to biopolymers and is very certified to write down approximately this topic. This e-book, although written mostly as a monograph of the author's personal contributions, can be a very good assessment of the topic. Of specific curiosity are the references to many vital Russian papers during this box that have now not been well-known within the Western literature. it's obvious from this booklet that fluorescence and phosphorescence equipment are getting used approximately as commonly in Russia as somewhere else on this planet and that we mustn't ever disregard those im portant contributions. Konev's reviews on protein fluorescence were widely known. it's of curiosity to benefit approximately those and different of his purposes. The final a part of the e-book, which bargains with fluorescence as a way to probe into the constitution and conforma tion of macromolecules in intact cells, is finest. apart from released symposia this e-book is the 1st written particularly approximately luminescence of biopolymers. Sidney Udenfriend Bethesda. Maryland might, 1967 v CONTENTS creation . • • . . . . . . . . . • . . . . . . . . . .
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Extra info for Fluorescence and Phosphorescence of Proteins and Nucleic Acids
13, curve 5). In fact, the polarization retains its high and constant positive values (about 25%) over the whole fluorescence band, except for the shortest-wave part. From 315 mflthere is a gradual reduction in the degree of polarization, which attains very low values (17%) in the region of 295 mfl-. The situation is different in a polar solvent, glycerol. where the fluorescence is only from the 1La level and where accordingly there is no reduction of polarization in the short-wave part of the fluorescence spectrum.
The 280-mf-L line, of course, excites a relatively larger proportion of 1Lb oscillators which have a phosphorescence component coinciding in direction with them. This leads to a reduction in the polarization at the corresponding points of the spectrum. When the 1La oscillator (only or predominantly) is excited, the oscillators of the two vibrational series of the phosphorescence band are oriented perpendicular to" it, which leads to constancy of the negative values of the polarization over the whole phosphorescence spectrum.
1 200 t' 02 -P,% Fig. 26. 1 N NaOH, respectively, at t =77°K (Vladimirov and Li Chin-kuo, 1962). 55 ELECTRONIC EXCITED STATES OF MONOMERS Table 3.