Download Engineering and Analyzing Multicellular Systems: Methods and by Lianhong Sun, Wenying Shou PDF

By Lianhong Sun, Wenying Shou

Engineering Multicellular structures: tools and Protocols, makes a speciality of laboratory systems utilized in contemporary efforts for developing man made multicellular platforms and their functions. particularly, developing multicellular structures to shape quite a few microbial ecosystems has been broadly explored to check evolution and interactions of microbial ecosystems, whereas co-cultures have emerged as an effective instrument to provide a few advanced chemical molecules. Written within the hugely winning Methods in Molecular Biology series layout, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols and key tips about troubleshooting and warding off recognized pitfalls.

Engineering Multicellular structures: equipment and Protocols supply a complete laboratory protocol reference for developing multicellular structures for numerous applications.

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Engineering and Analyzing Multicellular Systems: Methods and Protocols

Engineering Multicellular platforms: equipment and Protocols, specializes in laboratory approaches utilized in fresh efforts for developing man made multicellular platforms and their functions. specifically, developing multicellular structures to shape a number of microbial ecosystems has been broadly explored to envision evolution and interactions of microbial ecosystems, whereas co-cultures have emerged as a good instrument to provide a few complicated chemical molecules.

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Gill SR, Pop M, Deboy RT et al (2006) Metagenomic analysis of the human distal gut microbiome. Science 312:1355–1359 35. Bäckhed F, Ley RE, Sonnenburg JL et al (2005) Host-bacterial mutualism in the human intestine. Science 307:1915–1920 36. Guarner F, Malagelada J-R (2003) Gut flora in health and disease. Lancet 361:512–519 37. Stappenbeck TS, Hooper LV, Gordon JI (2002) Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells. Proc Natl Acad Sci U S A 99: 15451–15455 38.

In contrast, fluorescently tagged strains can be distinguished using flow cytometry, which allows tens of thousands of cells to be counted in less than a minute. While fluorescenceactivated cell sorting (FACS) can also be used to isolate subpopulations, it requires an expensive instrument and is less efficient when isolating very rare subpopulations. A large number of fluorescent proteins are available [14], although not all of them are bright and/or resolvable from one another using standard filter sets.

Interdiscip Perspect Infect Dis 2008:125081 66. Vrieze A, Holleman F, Zoetendal EG et al (2010) The environment within: how gut microbiota may influence metabolism and body composition. Diabetologia 53:606–613 67. Hou JK, Abraham B, El-Serag H (2011) Dietary intake and risk of developing inflammatory bowel disease: a systematic review of the literature. Am J Gastroenterol 106: 563–573 68. Fava F, Lovegrove JA, Gitau R et al (2006) The gut microbiota and lipid metabolism: implications for human health and coronary heart disease.

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