Download Current Trends in Histocompatibility: Volume 1 Immunogenetic by Jacques M. Colombani, Laurent Degos, Virginia Lepage, Hélène PDF

By Jacques M. Colombani, Laurent Degos, Virginia Lepage, Hélène Dastot, Muriel Reboul (auth.), Ralph A. Reisfeld, Soldano Ferrone (eds.)

Information approximately histocompatibility antigens is increasing so quickly that it truly is tough to stay abreast of aB advances. In those volumes, we've made an attempt to collect the most up-tp-date paintings on subject matters that experience generated many of the re cent advances and discussions. we now have requested every one writer to provide and interpret his most modern paintings, and we have now judiciously avoided implementing our personal prejudices and viewpoints. even supposing there's seen overlap in a few person issues, we've got inspired this to supply the reader with as many alternative and a few­ instances opposing viewpoints as attainable. This process will, we liope, supply a huge evaluate of present rules within the box. we want to thank all individuals for his or her well timed and fascinating manu­ scripts, and we essentially wish that the reader will make the most of those volumes. R. A. Reisfeld S. Ferrone l. a. Jolla ix Contents I. Serology and Genetics bankruptcy 1 reports of HLA-DR Antigens via supplement Fixation Jacques M. Colombani, Laurent Degos, Virginia Lepage, Helene Dastot, Muriel Reboul, and Pierre Lethielleux 1. creation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three 2. fabric and techniques. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three three. effects and dialogue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . four three. 1. Screening for Anti-HLA-DR Complement-Fixing Sera . . . . . . . . four three. 2. Expression of HLA-A, -B and -DR Antigens on Peripheral Blood Lymphocytes in the course of Phytohemagglutinin Stimulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 three. three. Expression of HLA-A, -B and -DR Antigens on Peripheral Blood Lymphocytes in the course of Concanavalin A Stimulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 three. 4.

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1.

Four clones were isolated and grown for further study. They were designated A2 ml, 2, 4, and 7. 1. Serology of the Mutant Cell Lines The SB parent line and the four c10ned variants have been extensively HLA typed by the standard NIH microcytotoxicity technique. Against a panel of 360 different antisera detecting HLA-A, -B, -C, and -DR antigens, all five cells typed identically except for five antisera. A1I 23 SEROLOGICAL AND CELLULAR RECOGNITION OF HLA ANTIGENS five of these sera were anti-HLA-A2 and all killed over 95% of the parent cell while failing to lyse the mutants.

Purified IgG from this absorbed antiserum was coupled to CNBr-activated Biogel A15m. 0 (DOCTS). 0, dialyzed vs. DOCTS, and concentrated by ultrafiltration using an Amicon XM-50 membrane. The starting material and the alkali-eluted material were examined for their HLA-A9, "w4," and Bw6 antigen content in an inhibition-of-complement-mediated-cytotoxicity assay using 51Cr-Iabeled PALA cells as targets. The results are shown in Table 2. 3% of the HLABw6 activity was present in this fraction. This is more evidence in favor of a molecular association between a w4-like determinant and HLAA9.

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