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G. f 1984; 1986) will inhibit adenylate cyclase without having a stimulatory effect on inositol lipid metabolism. Thus, at present it is not clear if G. couples receptors to phospholipase C in some cells but not in others. An alternative possibility is that there is a similar, as yet unidentified, G-binding protein that differs from G. in its susceptibility to ribosylation and inactivation by pertussis toxin in different cells, but which uniquely couples to phospholipase C in all cells. , 1985; Huff and Neer, 1986; Sternweis, 1986).

Proc. Natl. Acad. Sei. USA 7fi, 21B4. , and Belanger, L. (1985). Nucleic Acids Res. 13. 23B7. M. C1985b). Nature 31Br 670. , and Gorski, J. (1984). Nature 307r 747. , and Beato, M. (1980). Eur. J. Biochem. 106. 395. , and Beato, tl. (1982). ΕΜΒ0 J. 1, 1467. , and Gehring, U. (1984). ΕΜΒ0 J. 3, 1493. R. (19B4) ΕΠΒ0 J. 5, 2763. -A. (1984). J. Biol. Chem. , 4534. Chapter 2 SIGNALLING SYSTEMS IN STIMULUS-RESPONSE COUPLING1 John R. Williamson Carl A. Hansen Department of Biochemistry and Biophysics University of Pennsylvania Philadelphia, Pennsylvania 19104 I.

3. Japan Sei. Soc. Press/ Springer-Uerlag. Scheidereit, C , and Beato, M. C19B4D. Proc. Natl. Acad. Sei. USAfll,3029. , and Beato, M. C19B3D. Nature 3Q1, 749. , and Beato, M. C1996). DNA, in press. , and Berlin, C M . C1965). J. Biol. Chem. 240T 322. , and Lituack, G. C19B6). In "Glucocorticoid Hormones. Mechanism of Action" CY. Sakamoto and F. ), p. 35. Japan Sei. Soc. Press/Springer-Uerlag. O. C19B5) J. Biol. Chem. 260. 1492. , and Feigelson, P. (1973). Proc. Natl. Acad. Sei. USA 70, 1218. , and Schütz, ß.

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